Instruction of SigED


SigED is used to compute three Z-scores, SigZscre(Si), SigSteme(Si), and SigLoope(Si), of local segments in a sequence. These scores can be computed by sliding a fixed-length window in steps of a few nucleotides from 5' to 3' along the searched sequence. It is often used in a particular interested region that was previously determined by the more rapid method, edscan. The maximum sequence length is allowed upto 4995000 bp. In general the moving step size is 3-5 bp and segment (window) length is less than 300 bp.

The program SigED package (SigED.tar) includes a Readme file and files listed below:

1. Source Codes for SigED:

  • siged.f
  • tematf.f
  • trace.f
  • cfd.inc
  • 2. Energy Table Files for SigED:

  • turner.tbl (Turner energy rules for RNA folding)
  • It can be easily compiled by

  • f90 -o SigED -O3 -extend_source siged.f tematf.f trace.f
  • 3. A test job file: SigED.csh

    4. An input sequence file: eleg.seq

    5.An input control file: W75.in

    6. An output file: eleg_W75.zscr


    Back to SigED

    Please send questions or suggestions on this page to shuyun@ncifcrf.gov
    May 16, 2003